PRIMO is a micropatterning system mounted on a Nikon Ti2. It allows for the exquisite patterning and sculpting of cell substrate with a precision down to 2 micrometers. Further information is available from the Alveole lab website In the UK, PLPP reagent (liquid or gel) for PRIMO can be bought by contacting Cairn Research: Contact sales@cairn-research.co.uk Micropatterning: manipulating substrate composition Micropatterning relies on the specific degradation of an anti-fouling reagent to uncover specific area of the substrate. These areas, either plain or gradients, can then be coated with proteins. Seeded cells preferentially bind the protein-coated substrate. The composition of the substrate is therefore specifically manipulated. Image courtesy of Alveole Substrate sculpting: manipulating substrate shape PRIMO allows to polymerize, degrade or cut gels down to 2um precision. The shape of the substrate can therefore be designed. You can construct niches for your stem cells, labyrinth for chemotactic experiments, micro wells for seeding organoids. Image courtesy of Alveole Reproducible microfabrication of substrate: shape and composition By combining micropatterning and substrate sculpting, PRIMO provides complete control over your cell culture substrate. Image courtesy of Alveole Locations: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access This article was published on 2024-07-08
