Publications

A Single-Molecule Liposome Assay for Membrane Permeabilization. Bąk, Krzysztof M., Daniel C. Edwards, Dylan George, Bhanu Singh, Ryan Ferguson, Tianxiao Zhao, Kristin Piché, Ariel Louwrier, Scott L. Cockroft, and Mathew H. Horrocks. Angewandte Chemie (International Ed. in English), April 7, 2025, e202503678. https://doi.org/10.1002/anie.202503678.

Drug Combinations Targeting FAK and MEK Overcomes Tumor Heterogeneity in Glioblastoma. Furqan, Muhammad, Richard J. R. Elliott, Peter W. K. Nagle, John C. Dawson, Roza Masalmeh, Virginia Alvarez Garcia, Alison F. Munro, et al.  Pharmaceutics 17, no. 5 (April 23, 2025). https://doi.org/10.3390/pharmaceutics17050549.

Single-Cell Morphological Tracking of Cell States to Identify Small-Molecule Modulators of Liver Differentiation. Graham, Rebecca E., Runshi Zheng, Jesko Wagner, Asier Unciti-Broceta, David C. Hay, Stuart J. Forbes, Victoria L. Gadd, and Neil O. Carragher. IScience 28, no. 2 (February 21, 2025): 111871. https://doi.org/10.1016/j.isci.2025.111871.

Precision Molecular Engineering of Compact Near-Infrared Fluorophores. Huang, Rongrong, Qinglong Qiao, Deborah Seah, Tianruo Shen, Xia Wu, Fabio de Moliner, Chao Wang, et al. “ Journal of the American Chemical Society 147, no. 6 (February 12, 2025): 5258–68. https://doi.org/10.1021/jacs.4c16087.

Compound Screening in Human Airway Basal Cells Identifies Wnt Pathway Activators as Potential Pro-Regenerative Therapies. Ishii, Yuki, Jessica C. Orr, Marie-Belle El Mdawar, Denise R. Bairros de Pilger, David R. Pearce, Kyren A. Lazarus, Rebecca E. Graham, et al. Journal of Cell Science 138, no. 7 (April 1, 2025). https://doi.org/10.1242/jcs.263487.

Genomics-Led Approach to Drug Testing in Models of Undifferentiated Pleomorphic Sarcoma. Manasterski, Piotr J., Molly R. Danks, John P. Thomson, Morwenna Muir, Martin Lee, John C. Dawson, Ana T. Amaral, et al.  Molecular Oncology, May 26, 2025. https://doi.org/10.1002/1878-0261.70059.

Fluorogenic Platform for Real-Time Imaging of Subcellular Payload Release in Antibody-Drug Conjugates. Nadal-Bufi, Ferran, Paulin L. Salomon, Fabio de Moliner, Kathy A. Sarris, Zhi Wang, Rachel D. Wills, Violeta L. Marin, et al.  Journal of the American Chemical Society 147, no. 9 (March 5, 2025): 7578–87. https://doi.org/10.1021/jacs.4c16842.

Cellular Activity of CQWW Nullomer-Derived Peptides. Shave, Steven, Rebecka Isaksson, Nhan T. Pham, Richard J. R. Elliott, John C. Dawson, Julius Soudant, Neil O. Carragher, and Manfred Auer.  ACS Omega 10, no. 7 (February 25, 2025): 6794–6800. https://doi.org/10.1021/acsomega.4c08860.

An Encodable Amino Acid for Targeted Photocatalysis. Wong, Man Sing, Utsa Karmakar, Marco Bertolini, Abigail E. Reese, Lorena Mendive-Tapia, and Marc Vendrell.  Chemical Science 16, no. 10 (March 5, 2025): 4360–65. https://doi.org/10.1039/d4sc08594a.

Late-Stage Minimal Labeling of Peptides and Proteins for Real-Time Imaging of Cellular Trafficking. Nadal-Bufi F, Nithun RV, de Moliner F, Lin X, Habiballah S, Jbara M, Vendrell M. ACS Cent Sci. 2024; 11(1):66-75. https://doi.org/10.1021/acscentsci.4c01249. 

Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT. Bhaskar H, Gidden Z, Virdi G, Kleinjan DJ, Rosser SJ, Gandhi S, Regan L, Horrocks MH. Protein Sci. 2025; 34(2):e70008. https://doi.org/10.1002/pro.70008. 

A Long Fluorescence Lifetime Probe for Labeling of Gram-Negative Bacteria. Chem Biomed Imaging. Baibek A, Konieczna Z, Üçüncü M, Alghamdi ZS, Sharma R, Horrocks MH, Bradley M.. 2024; 3(1):45-50. https://doi.org/10.1021/cbmi.4c00066.

Aberrant Basement Membrane Production by HSCs in MASLD Is Attenuated by the Bile Acid Analog INT-767. Ramachandran, P.; Brice, M.; Sutherland, E. F.; Hoy, A. M.; Papachristoforou, E.; Jia, L.; Turner, F.; Kendall, T. J.; Marwick, J. A.; Carragher, N. O.; Oro, D.; Feigh, M.; Leeming, D. J.; Nielsen, M. J.; Karsdal, M. A.; Hartmann, N.; Erickson, M.; Adorini, L.; Roth, J. D.; Fallowfield, J. A.  Hepatol Commun 2024, 8 (12), e0574. https://doi.org/10.1097/HC9.0000000000000574.

Sequential Replacement of PSD95 Subunits in Postsynaptic Supercomplexes Is Slowest in the Cortex. Morris, K.; Bulovaite, E.; Kaizuka, T.; Schnorrenberg, S.; Adams, C. T.; Komiyama, N.; Mendive-Tapia, L.; Grant, S. G. N.; Horrocks, M. H.  Elife 2024, 13, RP99303. https://doi.org/10.7554/eLife.99303.

Targeting the PREX2/RAC1/PI3Kβ Signaling Axis Confers Sensitivity to Clinically Relevant Therapeutic Approaches in Melanoma. Ford, C. A.; Koludrovic, D.; Centeno, P. P.; Foth, M.; Tsonou, E.; Vlahov, N.; Sphyris, N.; Gilroy, K.; Bull, C.; Nixon, C.; Serrels, B.; Munro, A. F.; Dawson, J. C.; Carragher, N. O.; Pavet, V.; Hornigold, D. C.; Dunne, P. D.; Downward, J.; Welch, H. C.; Barry, S. T.; Sansom, O. J.; Campbell, A. D.  Cancer Res 2024. https://doi.org/10.1158/0008-5472.CAN-23-2814.

Late-Stage Minimal Labeling of Peptides and Proteins for Real-Time Imaging of Cellular Trafficking, F. Nadal-Bufi, R. V. Nithun, F. De Moliner, X. Lin, S. Habiballah, M. Jbara and M. Vendrell, ACS Cent. Sci., 2024, acscentsci.4c01249. http://doi.org/10.1021/acscentsci.4c01249

Label-Free SERS Sensors for Real-Time Monitoring of Tyrosine Phosphorylation, A. Geddis, L. Mendive-Tapia, A. Sujantho, E. Liu, S. McAughtrie, R. Goodwin, M. Vendrell and C. J. Campbell, Anal. Chem., 2024, 96, 17978–17983. http://doi.org/10.1021/acs.analchem.4c02860

Chemo-Click: Receptor-Controlled and Bioorthogonal Chemokine Ligation for Real-Time Imaging of Drug-Resistant Leukemic B Cells, M. Bertolini, L. Mendive-Tapia, U. Karmakar and M. Vendrell, J. Am. Chem. Soc., 2024, 146, 30565–30572. http://doi.org/10.1021/jacs.4c12035

Safety and efficacy of memantine and trazodone versus placebo for motor neuron disease (MND SMART): stage two interim analysis from the first cycle of a phase 3, multiarm, multistage, randomised, adaptive platform trial, S. Pal, J. Chataway, R. Swingler, M. R. Macleod, N. O. Carragher, G. Hardingham, B. T. Selvaraj, C. Smith, C. Wong, J. Newton, D. Lyle, A. Stenson, R. S. Dakin, A. Ihenacho, S. Colville, A. R. Mehta, N. Stallard, J. R. Carpenter, R. A. Parker, C. Keerie, C. J. Weir, B. Virgo, S. Morris, N. Waters, B. Gray, D. MacDonald, E. MacDonald, M. K. B. Parmar, S. Chandran, G. Gorrie, I. Morrison, C. Duncan, J. Carod Artal, T. Williams, V. Srinivasan, A. Radunovic, A. Pinto, H. Hamdalla, R. Roberts, P. Garcia- Reitboeck, T. Harrower, G. Mamutse, F. Crawley, C. Galton, K. Dawson, R. Forbes, C. Hillier, C. McDermott, D. Forbes, H. Bozkurt, M. Stavrou, P. Kearns, D. Breen, H. Kurucu King, E. Elliot, E. Beswick, J. Williamson, P. Cucurachi and L. McLennan, The Lancet Neurology, 2024, 23, 1097–1107. http://doi.org/10.1016/S1474-4422(24)00326-0

Rapid discovery and evolution of nanosensors containing fluorogenic amino acids, E. Kuru, J. Rittichier, H. De Puig, A. Flores, S. Rout, I. Han, A. E. Reese, T. M. Bartlett, F. De Moliner, S. G. Bernier, J. D. Galpin, J. Marchand, W. Bedell, L. Robinson-McCarthy, C. A. Ahern, T. G. Bernhardt, D. Z. Rudner, J. J. Collins, M. Vendrell and G. M. Church, Nat Commun, 2024, 15, 7531. http://doi.org/10.1038/s41467-024-50956-z

Tuning singlet oxygen generation with caged organic photosensitizers, E. Nestoros, F. De Moliner, F. Nadal-Bufi, D. Seah, M. C. Ortega-Liebana, Z. Cheng, S. Benson, C. Adam, L. Maierhofer, K. Kozoriz, J.-S. Lee, A. Unciti-Broceta and M. Vendrell, Nat Commun, 2024, 15, 7689. http://doi.org/10.1038/s41467-024-51872-y

An ILK/STAT3 pathway controls glioblastoma stem cell plasticity, A. E. P. Loftus, M. S. Romano, A. N. Phuong, B. J. McKinnel, M. T. Muir, M. Furqan, J. C. Dawson, L. Avalle, A. T. Douglas, R. L. Mort, A. Byron, N. O. Carragher, S. M. Pollard, V. G. Brunton and M. C. Frame, Developmental Cell, 2024, S1534580724005318. http://doi.org/10.1016/j.devcel.2024.09.003

The SRC family kinase inhibitor NXP900 demonstrates potent antitumor activity in squamous cell carcinomas, S. Dash, S. Hanson, B. King, K. Nyswaner, K. Foss, N. Tesi, M. J. B. Harvey, S. A. Navarro-Marchal, A. Woods, E. Poradosu, A. Unciti-Broceta, N. O. Carragher and J. Brognard, Journal of Biological Chemistry, 2024, 300, 107615. http://doi.org/10.1016/j.jbc.2024.107615

Photosensitizer‐Amplified Antimicrobial Materials for Broad‐Spectrum Ablation of Resistant Pathogens in Ocular Infections, C. Lochenie, S. Duncan, Y. Zhou, L. Fingerhut, A. Kiang, S. Benson, G. Jiang, X. Liu, B. Mills and M. Vendrell, Advanced Materials, 2024, 36, 2404107. http://doi.org/10.1002/adma.202404107

Highly Selective Drug-Derived Fluorescent Probes for the Cannabinoid Receptor Type 1 (CB₁ R), L. Mach, A. Omran, J. Bouma, S. Radetzki, D. A. Sykes, W. Guba, X. Li, C. Höffelmeyer, A. Hentsch, T. Gazzi, Y. Mostinski, M. Wasinska-Kalwa, F. De Molnier, C. Van Der Horst, J. P. Von Kries, M. Vendrell, T. Hua, D. B. Veprintsev, L. H. Heitman, U. Grether and M. Nazare, J. Med. Chem., 2024, 67, 11841–11867. http://doi.org/10.1021/acs.jmedchem.4c00465

Enzyme‐Activatable Near‐Infrared Hemicyanines as Modular Scaffolds for in vivo Photodynamic Therapy,  Z. Cheng, S. Benson, L. Mendive‐Tapia, E. Nestoros, C. Lochenie, D. Seah, K. Y. Chang, Y. Feng and M. Vendrell, Angew Chem Int Ed, 2024, 63, e202404587. http://doi.org/10.1002/anie.202404587

Sequential replacement of PSD95 subunits in postsynaptic supercomplexes is slowest in the cortex, K. Morris, E. Bulovaite, T. Kaizuka, S. Schnorrenberg, C. Adams, N. H. Komiyama, L. Mendive-Tapia, S. G. Grant and M. H. Horrocks, 2024, preprint, DOI: 10.7554/eLife.99303.1. http://doi.org/10.7554/eLife.99303.1

Multimodal decoding of human liver regeneration, K. P. Matchett, J. R. Wilson-Kanamori, J. R. Portman, C. A. Kapourani, F. Fercoq, S. May, E. Zajdel, M. Beltran, E. F. Sutherland, J. B. G. Mackey, M. Brice, G. C. Wilson, S. J. Wallace, L. Kitto, N. T. Younger, R. Dobie, D. J. Mole, G. C. Oniscu, S. J. Wigmore, P. Ramachandran, C. A. Vallejos, N. O. Carragher, M. M. Saeidinejad, A. Quaglia, R. Jalan, K. J. Simpson, T. J. Kendall, J. A. Rule, W. M. Lee, M. Hoare, C. J. Weston, J. C. Marioni, S. A. Teichmann, T. G. Bird, L. M. Carlin and N. C. Henderson, Nature, 2024, 630, 158–165. http://doi.org/10.1038/s41586-024-07376-2

RNA aptamer reveals nuclear TDP-43 pathology is an early aggregation event that coincides with STMN-2 cryptic splicing and precedes clinical manifestation in ALS, H. Spence, F. M. Waldron, R. S. Saleeb, A.-L. Brown, O. M. Rifai, M. Gilodi, F. Read, K. Roberts, G. Milne, D. Wilkinson, J. O’Shaughnessy, A. Pastore, P. Fratta, N. Shneider, G. G. Tartaglia, E. Zacco, M. H. Horrocks and J. M. Gregory, Acta Neuropathol, 2024, 147, 50. http://doi.org/10.1007/s00401-024-02705-1

Recent advances in minimal fluorescent probes for optical imaging, F. De Moliner, F. Nadal-Bufi and M. Vendrell, Current Opinion in Chemical Biology, 2024, 80, 102458. http://doi.org/10.1016/j.cbpa.2024.102458

SERS Microsensors for the Study of pH Regulation in Cystic Fibrosis Patient-Derived Airway Cultures, W. H. Skinner, N. Robinson, G. R. Hardisty, R. D. Gray and C. J. Campbell, ACS Sens., 2024, 9, 2550–2557. http://doi.org/10.1021/acssensors.4c00279

Brought to Light: A Fluorogenic Probe to Monitor Immunosuppressants, M. Werner and F. Thomas, ACS Cent. Sci., 2024, 10, 953–955. http://doi.org/10.1021/acscentsci.4c00665

Nonperturbative Fluorogenic Labeling of Immunophilins Enables the Wash-free Detection of Immunosuppressants, M. Bertolini, L. Mendive-Tapia, O. Ghashghaei, A. Reese, C. Lochenie, A. M. Schoepf, M. Sintes, K. Tokarczyk, Z. Nare, A. D. Scott, S. R. Knight, A. R. Aithal, A. Sachdeva, R. Lavilla and M. Vendrell, ACS Cent. Sci., 2024, 10, 969–977. http://doi.org/10.1021/acscentsci.3c01590

Selective Detection of Active Extracellular Granzyme A by Using a Novel Fluorescent Immunoprobe with Application to Inflammatory Diseases A. Senan-Salinas, L. Comas, P. Esteban, M. Garzón-Tituaña, Z. Cheng, L. Santiago, M. P. Domingo, A. Ramírez-Labrada, J. R. Paño-Pardo, M. Vendrell, J. Pardo, M. A. Arias and E. M. Galvez, ACS Pharmacol. Transl. Sci., 2024, 7, 1474–1484. http://doi.org/10.1021/acsptsci.4c00065

Novel dual antimicrobial-imaging photodynamic therapy agent labels and kills AMR gram-positive bacteria, A. Kiang, S. Benson, C. Lochenie, S. Duncan, S. M. P. C. Mohanan, G. O. S. Williams, K. Dhaliwal, M. Vendrell and B. Mills, in Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, SPIE, 2024, vol. PC12822, p. PC1282207. http://doi.org/10.1117/12.3001408

P017 An activity-based chemiluminescence assay targeting granzyme A for the detection of Inflammatory Bowel Diseases, J. Scott, E. Thompson, Z. Cheng, M. David, D. Gordon, L. Medive-Tapia, G. R. Jones, R. Hall, S. Chuah, B. Drury, A. Rossi, D. Shabat, G. T. Ho and M. Vendrell, Journal of Crohn’s and Colitis, 2024, 18, i267–i268. http://doi.org/10.1093/ecco-jcc/jjad212.0147

Inserting “OFF-to-ON” BODIPY Tags into Cytokines: A Fluorogenic Interleukin IL-33 for Real-Time Imaging of Immune Cells, A. E. Reese, F. De Moliner, L. Mendive-Tapia, S. Benson, E. Kuru, T. Bridge, J. Richards, J. Rittichier, T. Kitamura, A. Sachdeva, H. J. McSorley and M. Vendrell, ACS Cent. Sci., 2024, 10, 143–154. http://doi.org/10.1021/acscentsci.3c01125

Single-molecule imaging of aquaporin-4 array dynamics in astrocytes, A.-L. Zepernick, V. Metodieva, N. Pelegrina-Hidalgo, A. H. Lippert, M. H. Horrocks and J. A. Varela, Nanoscale, 2024, 16, 9576–9582. http://doi.org/10.1039/D4NR00330F

No-wash fluorogenic labeling of proteins for reversible photoswitching in live cells, K. Torii, S. Benson, Y. Hori, M. Vendrell and K. Kikuchi, Chem. Sci., 2024, 15, 1393–1401. http://doi.org/10.1039/D3SC04953A

Ligand-centred phenotype-driven development of potent kinase inhibitors against oesophageal cancer, C. C. Ayala-Aguilera, Y. Ge, Á. Lorente-Macías, B. N. Jones, C. Adam, N. O. Carragher and A. Unciti-Broceta, RSC Med. Chem., 2024, 10.1039.D4MD00579A. http://doi.org/10.1039/D4MD00579A

A cautionary note on the use of N-acetylcysteine as a reactive oxygen species antagonist to assess copper mediated cell death, R. E. Graham, R. J. R. Elliott, A. F. Munro and N. O. Carragher, PLoS ONE, 2023, 18, e0294297. http://doi.org/10.1371/journal.pone.0294297

Imaging Proteins Sensitive to Direct Fusions Using Transient Peptide–Peptide Interactions, Z. Gidden, C. Oi, E. J. Johnston, Z. Konieczna, H. Bhaskar, L. Mendive-Tapia, F. De Moliner, S. J. Rosser, S. G. J. Mochrie, M. Vendrell, M. H. Horrocks and L. Regan, Nano Lett., 2023, 23, 10633–10641. http://doi.org/10.1021/acs.nanolett.3c03780

Two-color coincidence single-molecule pulldown for the specific detection of disease-associated protein aggregates, R. S. Saleeb, C. Leighton, J.-E. Lee, J. O’Shaughnessy, K. Jeacock, A. Chappard, R. Cumberland, T. Zhao, S. R. Ball, M. Sunde, D. J. Clarke, K. Piché, J. A. McPhail, A. Louwrier, R. Angers, S. Gandhi, P. Downey, T. Kunath and M. H. Horrocks, Sci. Adv., 2023, 9, eadi7359. http://doi.org/10.1126/sciadv.adi7359

Fluorescent Probes for Imaging in Humans: Where Are We Now?, D. Seah, Z. Cheng and M. Vendrell, ACS Nano, 2023, 17, 19478–19490. http://doi.org/10.1021/acsnano.3c03564

Evolution and impact of high content imaging, G. P. Way, H. Sailem, S. Shave, R. Kasprowicz and N. O. Carragher, SLAS Discovery, 2023, 28, 292–305. http://doi.org/10.1016/j.slasd.2023.08.009