Slide scanners are widefield microscopes designed for routine imaging of large number of slides. Zeiss Axioscan Our colleagues from SURF host a number of Zeiss Axioscan. These slide scanners can image a large number of slides in brightfield or fluorescence (DAPI, GFP, RFP, Cy5). One Axioscan is capable of polarised microscopy, a technique used to observe the quasi-crystalline arrangement of biological macromolecules without staining, e.g. collagen fibres in fibrosis. The Axioscans are ideal for routine scanning of a large number of non-noisy samples. Contact SURF for access: irrslidescanning@ed.ac.uk Please complete this request form Location: IRR Histology Hub Akoya Polaris/Phenolmager We host the Akoya PhenoImager HT (formerly known as Vectra Polaris) on behalf of our colleagues from SURF. This slide scanner can image up to 80 slides in brightfield or fluorescence with 10x, 20x or 40x lenses. It is equipped with a liquid crystal tunable filter and a spectral camera that images samples across the whole light spectrum and can separate up to 9 colours, even if their spectra overlap (requires single dye controls as well as no dye control, unless using Opal dyes). The Polaris PhenoImager is ideal for noisy samples. Location: IRR Imaging Hub, contact irr-hcs@ed.ac.uk for access This article was published on 2024-07-08
