A widefield microscope is a camera-based system that takes an image of the whole field of view at once. It is very fast but sees objects above and below the focus point as noisy blur, which is not an issue for most thin samples like histological slices or cell monolayers. Widefield microscopes can have colour or greyscale cameras – the latter are ultra-sensitive and used for fluorescence microscopy. Expand all Collapse all Nikon Ti The Nikon Ti is a fully automated widefield microscope with an incubation chamber. It is equipped with an ultra-sensitive 16-bits greyscale camera and can image samples in brightfield/Phase as well as in fluorescence in DAPI, GFP, YFP, RFP, and Cy5. This microscope has long working distance lenses (10x, 20x, 40x) that can image through tissue culture plastic, as well as short working distance lenses (20x, 60x, 100x) for samples on coverslips or imaging quality substrate. Special applications and features: This microscope can run multiple positions, 3D stacks and timelapses. The addition of Nikon’s JOBS allows for extra flexibility in programming. YFP filter set –as long as you don’t have GFP! 100x lens on demand Location: First floor of IRR North (CRM Building), opposite the histology lab. Contact IRR.Imaging@ed.ac.uk for access Nikon Ti2 The Nikon Ti2 is a fully automated widefield microscope. It is equipped with an ultra-sensitive 16-bits greyscale camera and can image samples in brightfield/DIC as well as in fluorescence in DAPI, GFP, RFP, and Cy5. This microscope has long working distance lenses (5x, 10x, 20x) that can image through tissue culture plastic. Special applications and features: This microscope is used for substrate microfabrication using Alvéole’s PRIMO, as well as biomechanical measurement using OpticsLife11 Chiaro. Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access Zeiss Observer The Zeiss Observer is an automated widefield microscope. It is equipped with both a 12-bits colour camera and an ultra-sensitive 16-bits greyscale camera. This microscope can image classical histology and colorimetric dyed samples in brightfield. It can also image in phase as well as in fluorescence in DAPI, GFP, RFP, mPlum (Alexa 594) and Cy5. This microscope has long working distance lenses (5x, 10x, 20x,) that can image through tissue culture plastic, as well as short working distance lenses (20x, 40x, 60x) for samples on coverslips or imaging quality substrate. Special applications and features: This microscope can run multiple positions and 3D stacks. Greyscale (better for fluorescence) and colour cameras mPlum/Alexa 594 filter set Location: IRR Imaging Hub Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access Zeiss Observer Z1 Live The Zeiss Observer Z1 Live is a fully automated widefield microscope with an incubation chamber. It is equipped with both colour and ultra-sensitive 16-bits greyscale camera and can image samples in brightfield/Phase as well as in fluorescence in DAPI, GFP, RFP, mPlum (Alexa 594) and Cy5. The system is however very flexible and can detect more dyes, ask us for compatibility. This microscope has long working distance lenses (5x, 10x, 20x, 40x) that can image through tissue culture plastic, as well as short working distance lenses (20x, 60x) for samples on coverslips or imaging quality substrate. The Zen software allows for complete automation of timelapse. Special applications and features: This microscope can run multiple positions, 3D stacks and timelapse. Both colour and greyscale (better for fluorescence) cameras This microscope has multibandpass filters for extremely fast imaging of several fluorescence dyes. Combination of excitation and emission filters allow for flexible imaging of many more fluorophores. Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access Leica DMI8 The Leica DMI8 is an automated widefield microscope with a heated chamber, ideal for work with zebrafish and bacteria. It is equipped with both an RGB colour camera and an ultra-sensitive 16-bits greyscale camera and can image samples in brightfield/Phase as well as in fluorescence in DAPI, GFP, RFP, and Cy5. The LASX software allows for complete automation of timelapse. Special applications and features: This microscope can run multiple positions, 3D stacks and timelapse. Navigator scan for quick mapping with spiral option. The navigator allows for mark and find, as well as tiling of large and complex objects. Greyscale (better for fluorescence) and colour cameras Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access Leica upright DM 2500 The Leica DM2500 is a manual upright microscope with a colour camera. It is capable of imaging samples in fluorescence (DAPI, GFP, TXR, DsRed, Far red). This microscope is equipped with six lenses (5x, 10x, 20x, 40x, 63x and 100x). On request, we can supply 40x and 63x water immersion lenses that can be dipped in water. Special applications and features: This microscope is entirely manual. Colour camera Upright microscope (i.e. lens above specimen) with immersion lenses that can dip in watery medium, available on request. 100x lens, ideal for karyotyping, and a set of 40x and 63x dipping lenses ideal for wet work (e.g. zebrafish). Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access This article was published on 2024-07-08
