Publications from the Histology and Multiplexing facility Recent selected publications Rosario R, Stewart HL, Spears N, Telfer EE, Anderson RA. Anti-Mullerian hormone attenuates both cyclophosphamide-induced damage and PI3K signalling activation, while rapamycin attenuates only PI3K signalling activation, in human ovarian cortex in vitro. Hum Reprod. 2024 Feb 1;39(2):382-392. doi: 10.1093/humrep/dead255. Stephen West, Ammar Natalwala, Karamjit Singh Dolt, Douglas J. Lamont, Melanie McMillan, Kelvin Luk, Tomoji Mashimo, Tilo Kunath (2023). G51D mutation of the endogenous rat Snca gene disrupts synaptic localisation of α-synuclein priming for Lewy-like pathology. Biorxiv. doi: https://doi.org/10.1101/2023.10.27.564027 Rosario R, Stewart HL, Choudhury NR, Michlewski G, Charlet-Berguerand N, Anderson RA. Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency. FASEB J. 2022 Nov;36(11):e22612. doi: 10.1096/fj.202200468RR Marion Bonneau, et al. (2021) Functional brain defects in a mouse model of a chromosomal t(1;11) translocation that disrupts DISC1 and confers increased risk of psychiatric illness, Transl Psychiatry, 2021 Feb 19;11(1):135. doi: 10.1038/s41398-021-01256-3. Callum Arthurs, Alejandro Suarez-Bonnet, Claire Willis, Boyu Xie, Natalie Machulla, Tim S. Mair, Kevin Cao, Michael Millar, Christopher Thrasivoulou. (2021) Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2Journal of Pediatric Urology Volume 17, Issue 1, February 2021, Pages 100.e1-100.e10 Michael R Millar (2020) Mixed Multiplex Staining: Automated RNAscope™ and OPAL™ for Multiple Targets, Methods in molecular biology (Clifton, N.J.) 2148:277-298 DOI: 10.1007/978-1-0716-0623-0_18 In book: In Situ Hybridization Protocols Rosario R, Stewart HL, Walshe E, Anderson RA. Reduced retinoic acid synthesis accelerates prophase I and follicle activation. Reproduction. 2020 Sep;160(3):331-341. doi: 10.1530/REP-20-0221 Luca Cassetta, Stamatina Fragkogianni, Andrew H. Sims, Agnieszka Swierczak, Lesley M. Forrester, Hui Zhang, Daniel Y.H. Soong, Tiziana Cotechini, Pavana Anur, Elaine Y. Lin, Antonella Fidanza, Martha Lopez-Yrigoyen, Michael R. Millar, Alexandra Urman, Zhichao Ai, Paul T. Spellman, E. Shelley Hwang, J. Michael Dixon, Lisa Wiechmann, Lisa M. Coussens, Harriet O. Smith, and Jeffrey W. Pollard (2019), Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell https://doi.org/10.1016/j.ccell.2019.02.009 Full publications list - Lyndsey Boswell Reduced Transforming Growth Factor Beta Activity in the Endometrium of Women with Heavy Menstrual Bleeding Article January 2017 The Journal of Clinical Endocrinology and Metabolism In an Ovine Model of Polycystic Ovary Syndrome (PCOS) Prenatal Androgens Suppress Female Fetal Renal Gluconeogenesis July 2015 PLoS ONE Bone Morphogenetic Proteins Are Mediators of Luteolysis in the Human Corpus Luteum Article January 2015 Endocrinology Exogenous androgens reduce the expression of INSL3, a hormone involved in normal testicular descent, in fetal Leydig cells September 2014 Reproduction Abstracts The Loss of Luteal Progesterone Production in Women Is Associated With a Galectin Switch via α2,6-Sialylation of Glycoconjugates September 2014 The Journal of Clinical Endocrinology and Metabolism The pathophysiology of increased hepatic IGF1 expression in an ovine model of polycystic ovary syndrome Conference Paper May 2014. 16th European Congress of Endocrinology. Endocrine Transforming growth factor-[beta] superfamily signalling and its role in the pathogenesis of heavy menstrual bleeding March 2014 Endocrine Abstracts The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube February 2014. PLoS ONE Expression and localization of Inhibitor of Differentiation (ID) proteins during tissue and vascular remodelling in the human corpus luteum. November 2012 Molecular Human Reproduction SESSION 50: EARLY PREGNANCY - NEW EVIDENCE Article July 2012 Human Reproduction Why smoking is bad for your fallopian tube Conference Paper January 2012 28th Annual Meeting of the Human Reproduction Localization of the Inhibitor of Differentiation 1-4 (Id1-4) Transcription Factors in the Human Corpus Luteum across the Luteal Phase. July 2011. Biology of Reproduction. Hypoxia-Inducible Factor-1α Expression in Human Endometrium and Its Regulation by Prostaglandin E-Series Prostanoid Receptor 2 (EP2) March 2006. Endocrinology Full publications list - Melanie McMillan Stephen West, Ammar Natalwala, Karamjit Singh Dolt, Douglas J. Lamont, Melanie McMillan, Kelvin Luk, Tomoji Mashimo, Tilo Kunath (2023). G51D mutation of the endogenous rat Snca gene disrupts synaptic localisation of α-synuclein priming for Lewy-like pathology. Biorxiv. doi: https://doi.org/10.1101/2023.10.27.564027 Marion Bonneau, et al. (2021) Functional brain defects in a mouse model of a chromosomal t(1;11) translocation that disrupts DISC1 and confers increased risk of psychiatric illness, Transl Psychiatry, 2021 Feb 19;11(1):135. doi: 10.1038/s41398-021-01256-3. Full publications list - Mike Millar G. Kravvas, B. Xie, C. Thrasivoulou, M. Millar, A. Freeman, A. Haider, H. Alnajjar, A. Muneer, M. Lynch, C. Bunker (2023) 1044 Transcriptionally active human papillomavirus (HPV) in male genital lichen sclerosus (MGLSc), penile intraepithelial neoplasia (PeIN), and penile squamous cell carcinoma (PeSCC). Journal of Investigative Dermatology Volume 143, Issue 5, Supplement, May 2023, Page S179 B. Xie, N. Johal, M. Millar, C. Thrasivoulou, A.J. Kanai, A. Ahmed, C.H. Fry (2023) Quantification of the TGF- β /SMAD fibrosis pathway in bladder wall samples from children with congenital lower urinary tract anomalies. Continence Volume 5, March 2023, 100573 https://doi.org/10.1016/j.cont.2023.100573 Boyu Xie, Hawra Zuhair, Rui Henrique, Michael Millar, Timothy Robson, Christopher Thrasivoulou, Kerry Dickens, Jane Pendjiky, Asif Muneer, Hiten Patel, Aamir Ahmed (2023) Opposite changes in the expression of clathrin and caveolin‑1 in normal and cancerous human prostate tissue: putative clathrin‑mediated recycling of EGFR. Histochemistry and Cell Biology 2023, https://doi.org/10.1007/s00418-023-02183-8 Xie B, N. Johal , M. Millar , C. Thrasivoulou , A.J. Kanai , A. Ahmed , C.H. Fry (2023) Quantification of the TGF- β /SMAD fibrosis pathway in bladder wall samples from children with congenital lower urinary tract anomalies. Continence 2023, 5 https://doi.org/10.1016/j.cont.2023.100573 Boyu Xie, Hawra Zuhair, Rui Henrique, Michael Millar, Timothy Robson, Christopher Thrasivoulou, Kerry Dickens, Jane Pendjiky, Asif Muneer, Hiten Patel, Aamir Ahmed (2022) Opposite changes in the expression of clathrin and caveolin‑1in normal and cancerous human prostate tissue: putative clathrin‑mediated recycling of EGFR. Histochemistry and Cell Biology 159:489–500https://doi.org/10.1007/s00418-023-02183-8 Johal, N.; Cao, K.X.; Xie, B.; Millar, M.; Davda, R.; Ahmed, A.; Kanai, A.J.; Wood, D.N.; Jabr, R.I.; Fry, C.H. (2021) Contractile and Structural Properties of Detrusor from Children with Neurogenic Lower Urinary Tract Dysfunction. Biology 2021, 10, 863. https://doi.org/10.3390/ biology10090863 Callum Arthurs, Alejandro Suarez-Bonnet, Claire Willis, Boyu Xie, Natalie Machulla, Tim S. Mair, Kevin Cao, Michael Millar, Christopher Thrasivoulou. (2021) Equine penile squamous cell carcinoma: expression of biomarker proteins and EcPV2Journal of Pediatric Urology Volume 17, Issue 1, February 2021, Pages 100.e1-100.e10 Cristina Cosia, Michael Millar, Mariana Beltran, Lorcan Sherry, Silvia Gatti-McArthur.(2021) Quantitative analysis of RNAscope staining for c-fos expression in mouse brain tissue as a measure of Neuronal Activation, MethodsX Volume 8, 2021, 101348 Bushra Kanwal, Yoh Zen, Maesha Deheragoda, Michael Millar, Christopher Thrasivoulou, Sheikh Tamir Rashid, Aamir Ahmed (2020) Comparative analysis of Wnt signaling-related proteins in normal, benign, malignant and metastasised human liver tumors. bioRxiv preprint Nov 2020 https://doi.org/10.1101/2020.11.28.399865 Michael R Millar (2020) Mixed Multiplex Staining: Automated RNAscope™ and OPAL™ for Multiple Targets Methods in molecular biology (Clifton, N.J.) 2148:277-298 DOI: 10.1007/978-1-0716-0623-0_18 In book: In Situ Hybridization Protocols Navroop Johal, Kevin Cao, Callum Arthurs, Michael Millar, Christopher Thrasivoulou,Aamir Ahmed, Rita I. Jabr, Dan Wood, Peter Cuckow, Christopher H. Fry (2020) Contractile function of detrusor smooth muscle from children with posterior urethral valves – The role of fibrosis.(2020) Contractile function of detrusor smooth muscle from children with posterior urethral valves – The role of fibrosis. Scientific Reports volume 10, Article number: 7863 Luca Cassetta, Stamatina Fragkogianni, Andrew H. Sims, Agnieszka Swierczak, Lesley M. Forrester, Hui Zhang, Daniel Y.H. Soong, Tiziana Cotechini, Pavana Anur, Elaine Y. Lin, Antonella Fidanza, Martha Lopez-Yrigoyen, Michael R. Millar, Alexandra Urman, Zhichao Ai, Paul T. Spellman, E. Shelley Hwang, J. Michael Dixon, Lisa Wiechmann, Lisa M. Coussens, Harriet O. Smith, and Jeffrey W. Pollard (2019) Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets. Cancer Cell 2019, https://doi.org/10.1016/j.ccell.2019.02.009 Kevin Cao, Callum Arthurs, Ali Atta-ul, Michael Millar, Mariana Beltran, Jochen Neuhaus, Lars-Christian Horn, Rui Henrique, Aamir Ahmed and Christopher Thrasivoulou (2018) Quantitative Analysis of Seven New Prostate Cancer Biomarkers and the Potential Future of the ‘Biomarker Laboratory’ Diagnostics,8, 49; doi:10.3390/diagnostics8030049 www.mdpi.com/journal/diagnostics Smith JT, Roseweir A, Millar M, Clarke IJ, Millar RP (2018).Stimulation of growth hormone by kisspeptin antagonists in ewes (2018) Journal of Endocrinology 237(2):JOE-18-0074 DOI: 10.1530/JOE-18-0074 Callum Arthurs, Bibi Nazia Murtaza, Calum Thomson, Kerry Dickens, Rui Henrique, Hitendra R. H. Patel, MarianaBeltran, Michael Millar, Christopher Thrasivoulou, Aamir Ahmed (2017) Expression of ribosomal proteins in normal and cancerous human prostate tissue PLOS ONE| https://doi.org/10.1371/journal.pone.0186047October10,201714/14 Antonio Giuliano, Rebecca Swift, Callum Arthurs, Georgina Marote, Francesca Abramo, Jenny McKay, Calum Thomson, Mariana Beltran, Michael Millar, Simon Priestnall, Jane Dobson, Fernando Costantino-Casas, Terry Petrou, Imelda M. McGonnell, Anthony J. Davies, Malcolm Weetman, Oliver A. Garden, John R. Masters, Christopher Thrasivoulou, Aamir Ahmed (2016) Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma. PLoS ONE 11(8): e0161103.doi:10.1371/journal.pone.0161103 S. Plummer, M. Beltran, M. Millar, R. Currie and J. Wright (2015) Exploring AOP's and biomarkers of carcinogenesis in rats using laser dissection targeted (LDT) microarray profiling and pathways analysis (2016). Toxicology Letters CallumArthurs, Bibi Nazia Murtaza, Calum Thomson, Kerry Dickens ,Rui Henrique, Hitendra R.H.Patel,, Mariana Beltran, Michael Millar,Christopher Thrasivoulou, Aamir Ahmed. Expression of ribosomal proteins in normal and cancerous human prostate tissue (2016) PLoS ONE 12(10): e0186047. https://doi.org/10.1371/journal.pone.0186047 Manit Arya, Christopher Thrasivoulou, Rui Henrique, Michael Millar, Ruth Hamblin, Reena Davda, Kristina Aare, John R Masters, Calum Thomson, Asif Muneer, Hitendra R H Patel, Aamir Ahmed: Targets of Wnt/ß-Catenin Transcription in Penile Carcinoma (2016) PLoS ONE 04/2015; 10(4):e0124395. DOI:10.1371/journal.pone.0124395 Andrew J Symes, Marte Eilertsen, Michael Millar, Joseph Nariculam, Alex Freeman, Maria Notara, Mark R Feneley, Hitenedra R H Patel, John R W Masters, Aamir Ahmed: Quantitative analysis of BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer. PLoS ONE 12/2013; 8(12):e84295. DOI:10.1371/journal.pone.0084295 Christopher Thrasivoulou, Michael Millar, Aamir Ahmed: Activation of Intracellular Calcium by Multiple Wnt Ligands and Translocation of β-Catenin into the Nucleus. Journal of Biological Chemistry 10/2013; 288(50). DOI:10.1074/jbc.M112.437913 Nikhil Vilas Joshi, Jennifer B Raftis, Andrew J Lucking, Amanda H Hunter, Mike Millar, Mike Fitzpatrick, Giora Z Feuerstein, David E Newby: Lyophilised reconstituted human platelets increase thrombus formation in a clinical ex vivo model of deep arterial injury. Thrombosis and Haemostasis 05/2012; 108(1):176-82. DOI:10.1160/TH12-02-0059 Dganit Dinour, Nicola K Gray, Susan Campbell, Xinhua Shu, Lindsay Sawyer, William Richardson, Gideon Rechavi, Ninette Amariglio, Liat Ganon, Ben-Ami Sela, Hilla Bahat, Michael Goldman, Joshua Weissgarten, Michael R Millar, Alan F Wright, Eliezer J Holtzman: Homozygous SLC2A9 Mutations Cause Severe Renal Hypouricemia. Journal of the American Society of Nephrology 11/2009; 21(1):64-72. DOI:10.1681/ASN.2009040406 Bronwen Martin, Stuart Maudsley, Judith McNeilly, Linda Nicol, Janet Crawford, Michael Millar, Richard M Sharpe, Alan S McNeilly: Neonatal estrogenic effects upon the male rat pituitary: Early gonadotrophin attenuation precedes long-term recovery. Neuromolecular medicine 07/2009; 11(2):76-86. DOI:10.1007/s12017-009-8075-0 Zvi Naor, Henry N Jabbour, Michal Naidich, Adam J Pawson, Kevin Morgan, Sharon Battersby, Michael R Millar, Pamela Brown, Robert P Millar: Reciprocal Cross Talk between Gonadotropin-Releasing Hormone (GnRH) and Prostaglandin Receptors Regulates GnRH Receptor Expression and Differential Gonadotropin Secretion. Molecular Endocrinology 03/2007; 21(2):524-37. DOI:10.1210/me.2006-0253 Caroline Pope, Judy R McNeilly, Shiona Coutts, Mike Millar, Richard A Anderson, Alan S McNeilly: Gonadotrope and thyrotrope development in the human and mouse anterior pituitary gland. Developmental Biology 10/2006; 297(1):172-81. DOI:10.1016/j.ydbio.2006.05.005 Jayne E Sierens, Sharon F Sneddon, Frances Collins, Michael R Millar, Philippa T K Saunders: Estrogens in Testis Biology. Annals of the New York Academy of Sciences 01/2006; 1061(1):65-76. DOI:10.1196/annals.1336.008 Philippa T. K. Saunders, Jayne E. Sierens, Niegel P. Groome, Michael R. Millar: Les récepteurs aux œstrogènes dans le testicule et l’appareil reproducteur du primate et de l’homme. Andrologie 03/2003; 13(1):34-42. DOI:10.1007/BF03034407 Philippa T. K. Saunders, Jayne E. Sierens, Niegel P. Groome, Michael R. Millar: Oestrogen receptors in the human and primate testis and reproductive tract. Andrologie 03/2003; 13(1):43-50. DOI:10.1007/BF03034408 Kan-Tai Hsia, Michael R Millar, Sasha King, Jim Selfridge, Nicola J Redhead, David W Melton, Philippa T K Saunders: DNA repair gene Ercc1 is essential for normal spermatogenesis and oogenesis and for functional integrity of germ cell DNA in the mouse. Development 02/2003; 130(2):369-78. Graeme A Scobie, Sheila Macpherson, Michael R Millar, Nigel P Groome, Panagiota G Romana, Philippa T K Saunders: Human estrogen receptors : differential expression of ERalpha and beta and the identification of ERbeta variants. Steroids 12/2002; 67(12):985-92. DOI:10.1016/S0039-128X(02)00047-8 Philippa T K Saunders, Michael R Millar, Sheila Macpherson, D Stewart Irvine, Nigel P Groome, Lee R Evans, Richard M Sharpe, Graeme A Scobie: ERbeta1 and the ERbeta2 splice variant (ERbetacx/beta2) are expressed in distinct cell populations in the adult human testis.. Journal of Clinical Endocrinology & Metabolism 07/2002; 87(6):2706-15. Philippa T. K. Saunders, Michael R. Millar, Sheila Macpherson, D. Stewart Irvine, Nigel P. Groome, Lee R. Evans, Richard M. Sharpe, Graeme A. Scobie: ERβ1 and the ERβ2 Splice Variant (ERβcx/β2) Are Expressed in Distinct Cell Populations in the Adult Human Testis. Journal of Clinical Endocrinology & Metabolism 06/2002; 87(6):2706-2715. DOI:10.1210/jcem.87.6.8619 Jacqui Piner, Mark Sutherland, Mike Millar, Katie Turner, Derek Newall, Richard M Sharpe: Changes in vascular dynamics of the adult rat testis leading to transient accumulation of seminiferous tubule fluid after administration of a novel 5-hydroxytryptamine (5-HT) agonist. Reproductive Toxicology 03/2002; 16(2):141-50. DOI:10.1016/S0890-6238(02)00008-4 K J Turner, S Macpherson, M R Millar, A S McNeilly, K Williams, M Cranfield, N P Groome, R M Sharpe, H M Fraser, P T K Saunders: Development and validation of a new monoclonal antibody to mammalian aromatase. Journal of Endocrinology 02/2002; 172(1):21-30. P T K Saunders, M R Millar, K Williams, S Macpherson, C Bayne, C O'Sullivan, T J Anderson, N P Groome, W R Miller: Expression of oestrogen receptor beta (ERβI) protein in human breast cancer biopsies. British Journal of Cancer 02/2002; 86(2):250-6. DOI:10.1038/sj.bjc.6600035 Philippa T. K. Saunders, Michael R. Millar, Sheila MacPherson, D. Stewart Irvine, Nigel P. Groome, Lee R. Evans, Richard M. Sharpe, Greame A. Scobie: ER1 and the ER2 Splice Variant (ERcx/2) Are Expressed in Distinct Cell Populations in the Adult Human Testis. RP Millar, S Lowe, D Conklin, A Pawson, S Maudsley, B Troskie, T Ott, M Millar, G Lincoln, R Sellar, B Faurholm, G Scobie, R Kuestner, E Terasawa, A Katz: A novel mammalian receptor for the evolutionarily conserved type II GnRH. Proceedings of the National Academy of Sciences 09/2001; 98(17):9636-41. DOI:10.1073/pnas.141048498 Katie J Turner, Michelle Morley, Sheila MacPherson, Michael R Millar, Julie A Wilson, Richard M Sharpe, Philippa T.K Saunders: Modulation of gene expression by androgen and estrogen in the testis and prostate of the adult rat following androgen withdrawal. Molecular and Cellular Endocrinology 07/2001; 178(1-178):73-87. DOI:10.1016/S0303-7207(01)00413-0 Philippa T.K. Saunders, Richard M. Sharpe, Karin Williams, Sheila Macpherson, Holly Urquart, D.Stewart Irvine, Michael R. Millar: Differential expression of oestrogen receptor α and β proteins in the testes and male reproductive system of human and non-human primates. Molecular Human Reproduction 04/2001; 7(3):227-36. DOI:10.1093/molehr/7.3.227 Hilary O. D. Critchley, Robert M. Brenner, TA Henderson, Karin Williams, Nihar R. Nayak, Ov D. Slayden, Michael R. Millar, Philippa T. K. Saunders: Estrogen Receptor β, But Not Estrogen Receptor α, Is Present in the Vascular Endothelium of the Human and Nonhuman Primate Endometrium1. Journal of Clinical Endocrinology & Metabolism 04/2001; 86(3):1370-8. DOI:10.1210/jcem.86.3.7317 N Atanassova, C McKinnell, K Williams, K J Turner, J S Fisher, P. T. K. Saunders, M R Millar, R M Sharpe: Age-, Cell- and Region-Specific Immunoexpression of Estrogen Receptor α (But Not Estrogen Receptor β) during Postnatal Development of the Epididymis and Vas Deferens of the Rat and Disruption of This Pattern by Neonatal Treatment with Diethylstilbestrol1. Endocrinology 03/2001; 142(2):874-86. DOI:10.1210/en.142.2.874 P.T.K. Saunders, M R Millar, K Williams, S Macpherson, D Harkiss, R A Anderson, B Orr, N P Groome, G Scobie, H M Fraser: Differential expression of estrogen receptor-α and -β and androgen receptor in the ovaries of marmosets and humans. Biology of Reproduction 11/2000; 63(4):1098-105. Michael R. Millar, Richard M. Sharpe, Gerhard F. Weinbauer, Hamish M. Fraser, Philippa T. K. Saunders: Marmoset spermatogenesis: Organizational similarities to the human. International Journal of Andrology 11/2000; 23(5):266-77. DOI:10.1046/j.1365-2605.2000.00236.x N Atanassova, C McKinnell, K J Turner, MJ Walker, J S Fisher, M Morley, M R Millar, N P Groome, R M Sharpe: Comparative Effects of Neonatal Exposure of Male Rats to Potent and Weak (Environmental) Estrogens on Spermatogenesis at Puberty and the Relationship to Adult Testis Size and Fertility: Evidence for Stimulatory Effects of Low Estrogen Levels 1. Endocrinology 11/2000; 141(10):3898-907. DOI:10.1210/endo.141.10.7723 R M Sharpe, M Walker, M R Millar, N Atanassova, K Morris, C McKinnell, P.T.K. Saunders, H M Fraser: Effect of Neonatal Gonadotropin-Releasing Hormone Antagonist Administration on Sertoli Cell Number and Testicular Development in the Marmoset: Comparison with the Rat. Biology of Reproduction 07/2000; 62(6):1685-93. DOI:10.1095/biolreprod62.6.1685 K Williams, P.T.K Saunders, N Atanassova, J S Fisher, K J Turner, M R Millar, C McKinnell, R M Sharpe: Induction of progesterone receptor immunoexpression throughout the male reproductive tract after neonatal estrogen treatment of rats. Molecular and Cellular Endocrinology 07/2000; 164(1-2):117-31. DOI:10.1016/S0303-7207(00)00231-8 N Atanassova, C McKinnell, M Walker, K J Turner, J S Fisher, M Morley, M R Millar, N P Groome, R M Sharpe: Permanent Effects of Neonatal Estrogen Exposure in Rats on Reproductive Hormone Levels, Sertoli Cell Number, and the Efficiency of Spermatogenesis in Adulthood 1. Endocrinology 11/1999; 140(11):5364-73. DOI:10.1210/en.140.11.5364 R M Sharpe, K J Turner, C McKinnell, N P Groome, N Atanassova, M R Millar, D L Buchanan, P S Cooke: Inhibin B Levels in Plasma of the Male Rat from Birth to Adulthood: Effect of Experimental Manipulation of Sertoli Cell Number. Journal of Andrology 02/1999; 20(1):94-101. DOI:10.1002/j.1939-4640.1999.tb02501.x R M Sharpe, N. N. Atanassova, C McKinnell, P Parte, K J Turner, J S Fisher, J B Kerr, N P Groome, S Macpherson, M R Millar, P.T.K. Saunders: Abnormalities in functional development of the Sertoli cells in rats treated neonatally with diethylstilbestrol: A possible role for estrogens in Sertoli cell development. Biology of Reproduction 12/1998; 59(5):1084-94. DOI:10.1095/biolreprod59.5.1084 P.T.K. Saunders, J S Fisher, R M Sharpe, M R Millar: Expression of oestrogen receptor beta (ESR2) occurs in multiple cell types, including some germ cells, in the rat testis. Journal of Endocrinology 04/1998; 156(3):R13-7. DOI:10.1677/joe.0.156R013 K J Turner, R M Sharpe, J Gaughan, M R Millar, P M Foster, P T Saunders: Expression cloning of a rat testicular transcript abundant in germ cells, which contains two leucine zipper motifs.. Biology of Reproduction 12/1997; 57(5):1223-32. P.T.K. Saunders, S M Maguire, J Gaughan, M R Millar: Expression of oestrogen receptor beta (ER beta) in multiple rat tissues. Journal of Endocrinology 10/1997; 154(3):R13-6. DOI:10.1677/joe.0.154R013 S M Maguire, M R Millar, R M Sharpe, J Gaughan, P T K Saunders: Investigation of the potential role of the germ cell complement in control of the expression of transferrin mRNA in the prepubertal and adult rat testis. Journal of Molecular Endocrinology 09/1997; 19(1):67-77. DOI:10.1677/jme.0.0190067 J S Fisher, M R Millar, G Majdic, P T K Saunders, H M Fraser, R M Sharpe: Immunolocalisation of oestrogen receptor-α (ERα) within the testis and excurrent ducts of the rat and marmoset monkey from perinatal life to adulthood. Journal of Endocrinology 07/1997; 153(3):485-95. DOI:10.1677/joe.0.1530485 D J Elliott, M R Millar, K Oghene, A Ross, F Kiesewetter, J Pryor, M McIntyre, T B Hargreave, P. T. K. Saunders, P H Vogt, A C Chandley, H Cooke: Elliott, D.J. et al. Expression of RBM in the nuclei of human germ cells is dependent on a critical region of the Y chromosome long arm. Proc. Natl Acad. Sci. USA 94, 3848-3853. Proceedings of the National Academy of Sciences 05/1997; 94(8):3848-53. DOI:10.1073/pnas.94.8.3848 T Sweeney, P T Saunders, M R Millar, A N Brooks: Ontogeny of anti-müllerian hormone, 3 beta-hydroxysteroid dehydrogenase and androgen receptor expression during ovine total gonadal development.. Journal of Endocrinology 05/1997; 153(1):27-32. T Sweeney, P T K Saunders, M R Millar, A N Brooks: Ontogeny of anti-Mullerian hormone, 3 -hydroxysteroid dehydrogenase and androgen receptor expression during ovine fetal gonadal development. Journal of Endocrinology 04/1997; 153(1):27-32. DOI:10.1677/joe.0.1530027 P.T.K. Saunders, G Majdic, P Parte, M R Millar, J S Fisher, K J Turner, R M Sharpe: Fetal and Perinatal Influence of Xenoestrogens on Testis Gene Expression. Advances in Experimental Medicine and Biology 02/1997; 424:99-110. DOI:10.1007/978-1-4615-5913-9_19 P. T. K. Saunders, J Gaughan, B A Saxty, L E Kerr, M R Millar: Expression of protamine P2 in the testis of the common marmoset and man visualized using non-radioactive in-situ hybridization. International Journal of Andrology 09/1996; 19(4):212-9. DOI:10.1111/j.1365-2605.1996.tb00465.x M R Millar, R M Sharpe, S M Maguire, J Gaughan, P. T. K. Saunders: Co-localisation of mRNA and protein using in-situ hybridization and immunocytochemistry in testicular tissue. Microscopy Research and Technique 12/1995; 32(6):498-503. DOI:10.1002/jemt.1070320603 G Majdic, M R Millar, P T K Saunders: Immunolocalisation of androgen receptor to interstitial cells in fetal rat testes and to mesenchymal and epithelial cells of associated ducts. Journal of Endocrinology 12/1995; 147(2):285-93. DOI:10.1677/joe.0.1470285 Richard M. Sharpe, Janes S. Fisher, Mike M. Millar, Susan Jobling, John P. Sumpter: Gestational and Lactational Exposure of Rats to Xenoestrogens Results in Reduced Testicular Size and Sperm Production. Environmental Health Perspectives 12/1995; 103(12):1136-43. DOI:10.1289/ehp.951031136 R.M. Sharpe, S.M. Maguire, P.T. Saunders, M.R. Millar, L.D. Russell, D. Ganten, S. Bachmann, L. Mullins, J.J. Mullins: Infertility in a transgenic rat due to impairment of cytoplasmic elimination and sperm release from the Sertoli cells [corrected and republished with original paging, article originally printed in Biol Reprod 1995 Jul;53(1):214-26]. Biology of Reproduction 08/1995; 53:214-226. M Tetsuka, P F Whitelaw, W J Bremner, M R Millar, C D Smyth, S G Hillier: Developmental regulation of androgen receptor in rat ovary. Journal of Endocrinology 07/1995; 145(3):535-43. DOI:10.1677/joe.0.1450535 R M Sharpe, S M Maguire, P.T.K. Saunders, M R Millar, L D Russell, D Ganten, S Bachmann, L Mullins, J J Mullins: Infertility in a transgenic rat due to impairment of cytoplasmic elimination and sperm release from the Sertoli cells. Biology of Reproduction 07/1995; 53(1):214-26. DOI:10.1095/biolreprod53.2.214 P.T.K. Saunders, C. M. McKinnell, M.R. Millar, J Gaughan, K.J. Turner, B Jégou, V Syed, R.M. Sharpe: Phosphotidylethanolamine binding protein is an abudant secretory product of haploid testicular germ cells in the rat. Molecular and Cellular Endocrinology 03/1995; 107(2):221-30. DOI:10.1016/0303-7207(94)03447-2 Mike Millar: Sharpe, RM. , Fisher, JS. , Millar, MM. , Jobling, S. and Sumpter, JP. (1995) 'Gestational and lactational exposure of rats to xenoestrogens results in reduced testicular size and sperm production'. Environmental Health Perspectives, 103 (12). pp. 1136 - 1143.. W J Bremner, M R Millar, R M Sharpe, P.T.K. Saunders: Immunohistochemical localization of androgen receptors in the rat testis: Evidence for stage-dependent expression and regulation by androgens. Endocrinology 10/1994; 135(3):1227-34. DOI:10.1210/endo.135.3.8070367 M R Millar, R M Sharpe, S M Maguire, J Gaughan, A P West, P. T. K. Saunders: Localization of mRNAs by in-situ hybridization to the residual body at stages IX-X of the cycle of the rat seminiferous epithelium: Fact or artefact?. International Journal of Andrology 07/1994; 17(3):149-60. DOI:10.1111/j.1365-2605.1994.tb01235.x R M Sharpe, J B Kerr, C McKinnell, M Millar: Temporal relationship between androgen-dependent changes in the volume of seminiferous tubule fluid, lumen size and seminiferous tubule protein secretion in rats. J Reprod Fertil 06/1994; 101(1):193-8. DOI:10.1530/jrf.0.1010193 H L Ang, R Ivell, N Walther, H Nicholson, H Ungefroren, M Millar, Carter DA, D Murphy: Over-expression of oxytocin in the testes of a transgenic mouse model. Journal of Endocrinology 02/1994; 140(1):53-62. DOI:10.1677/joe.0.1400053 J B Kerr, G N Savage, M Millar, R M Sharpe: Response of the seminiferous epithelium of the rat testis to withdrawal of androgen: evidence for direct effect upon intercellular spaces associated with Sertoli cell junctional complexes. Cell and Tissue Research 11/1993; 274(1):153-61. DOI:10.1007/BF00327996 M R Millar, R M Sharpe, S M Maguire, P. T. K. Saunders: Cellular localization of messenger RNAs in rat testis: Application of digoxigenin-labelled probes to embedded tissue. Cell and Tissue Research 09/1993; 273(2):269-77. DOI:10.1007/BF00312828 S M Maguire, M R Millar, R M Sharpe, P.T.K. Saunders: Stage-dependent expression of mRNA for cyclic protein 2 during spermatogenesis is modulated by elongate spermatids. Molecular and Cellular Endocrinology 08/1993; 94(1):79-88. DOI:10.1016/0303-7207(93)90054-N W X Wu, J Brooks, M R Millar, W L Ledger, A F Glasier, A S McNeilly: Immunolocalization of oestrogen and progesterone receptors in the human decidua in relation to prolactin production. Human Reproduction 08/1993; 8(7):1129-35. J B Kerr, M Millar, S Maddocks, R M Sharpe: Stage-dependent changes in spermatogenesis and Sertoli cells in relation to the onset of spermatogenic failure following withdrawal of testosterone. The Anatomical Record 04/1993; 235(4):547-59. DOI:10.1002/ar.1092350407 Richard M. Sharpe, Mike Millar, Chris Mckinnell: Relative roles of testosterone and the germ cell complement in determining stage-dependent changes in protein secretion by isolated rat seminiferous tubules. International Journal of Andrology 03/1993; 16(1):71-81. DOI:10.1111/j.1365-2605.1993.tb01155.x P T Saunders, M R Millar, A P West, R M Sharpe: Mitochondrial cytochrome C oxidase II messenger ribonucleic acid is expressed in pachytene spermatocytes at high levels and in a stage-dependent manner during spermatogenesis in the rat.. Biology of Reproduction 02/1993; 48(1):57-67. P. T. K. Saunders, M R Millar, S M Maguire, R M Sharpe: Stage specific expression of rat transition protein 2 mRNA and localisation to the chroniatoid body of step 7 sper-matids by in situ hybridisation using a non-radioactive ribo-probe. Molecular Reproduction and Development 12/1992; 33(4):385-91. DOI:10.1002/mrd.1080330404 I A Greer, M Millar, A A Calder: Gemeprost-induced cervical ripening: histological and biophysical effects. European Journal of Obstetrics & Gynecology and Reproductive Biology 10/1992; 47(1):1-9. DOI:10.1016/0028-2243(92)90207-F R M Sharpe, S Maddocks, Millar MM, J B Kerr, P.T.K. Saunders, C McKinnell: Testosterone and spermatogenesis: Identification of stage-specific, androgen-regulated proteins secreted by adult rat seminiferous tubules. Journal of Andrology 03/1992; 13(2):172-84. DOI:10.1002/j.1939-4640.1992.tb01653.x W X Wu, J Brooks, M R Millar, W L Ledger, P. T. K. Saunders, AF Glasier, A S McNeilly: Localization of the sites of synthesis and action of prolactin by immunocytochemistry and in situ hybridization within the human utero-placental unit. Journal of Molecular Endocrinology 01/1992; 7(3):241-7. DOI:10.1677/jme.0.0070241 K B Smith, M R Millar, A S McNeilly, P J Illingworth, H M Fraser, D T Baird: Immunocytochemical localization of inhibin α-subunit in the human corpus luteum. Journal of Endocrinology 05/1991; 129(1):155-60. K. B. Smith, M. R. Millar, A. S. McNeilly, P. J. Illingworth, H. M. Fraser, D. T. Baird: Immunocytochemical localization of inhibin -subunit in the human corpus luteum. Journal of Endocrinology 04/1991; 129(1). DOI:10.1677/joe.0.1290155 Full publications list - Hazel Stewart Rosario R, Stewart HL, Spears N, Telfer EE, Anderson RA. Anti-Mullerian hormone attenuates both cyclophosphamide-induced damage and PI3K signalling activation, while rapamycin attenuates only PI3K signalling activation, in human ovarian cortex in vitro. Hum Reprod. 2024 Feb 1;39(2):382-392. doi: 10.1093/humrep/dead255. Rosario R, Stewart HL, Choudhury NR, Michlewski G, Charlet-Berguerand N, Anderson RA. Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency. FASEB J. 2022 Nov;36(11):e22612. doi: 10.1096/fj.202200468RR Rosario R, Stewart HL, Walshe E, Anderson RA. Reduced retinoic acid synthesis accelerates prophase I and follicle activation. Reproduction. 2020 Sep;160(3):331-341. doi: 10.1530/REP-20-0221 Rosario R, Crichton JH, Stewart HL, Childs AJ, Adams IR, Anderson RA. Dazl determines primordial follicle formation through the translational regulation of Tex14. FASEB J. 2019 Dec;33(12):14221-14233 doi: 10.1096/fj.201901247R. Epub 2019 Oct 26. Rosario R, Filis P, Tessyman V, Kinnell H, Childs AJ, Gray NK, Anderson RA. FMRP Associates with Cytoplasmic Granules at the Onset of Meiosis in the Human Oocyte. PLoS One. 2016 Oct 3;11(10):e0163987. doi: 10.1371/journal.pone.0163987. eCollection 2016. Bayne RA, Donnachie DJ, Kinnell HL, Childs AJ, Anderson RA. BMP signalling in human fetal ovary somatic cells is modulated in a gene-specific fashion by GREM1 and GREM2. Mol Hum Reprod. 2016 Sep;22(9):622-33. doi: 10.1093/molehr/gaw044. Epub 2016 Jul 6. Dean A, van den Driesche S, Wang Y, McKinnell C, Macpherson S, Eddie SL, Kinnell H, Hurtado-Gonzalez P, Chambers TJ, Stevenson K, Wolfinger E, Hrabalkova L, Calarrao A, Bayne RA, Hagen CP, Mitchell RT, Anderson RA, Sharpe RM. Analgesic exposure in pregnant rats affects fetal germ cell development with inter-generational reproductive consequences. Sci Rep. 2016 Jan 27;6:19789. doi: 10.1038/srep19789. Anderson RA, McIlwain L, Coutts S, Kinnell HL, Fowler PA, Childs AJ. Activation of the aryl hydrocarbon receptor by a component of cigarette smoke reduces germ cell proliferation in the human fetal ovary. Mol Hum Reprod. 2015 Sep;21(9):753. doi: 10.1093/molehr/gav044. Eddie SL, Childs AJ, Kinnell HL, Brown P, Jabbour HN, Anderson RA. Prokineticin Ligands and Receptors Are Expressed in the Human Fetal Ovary and Regulate Germ Cell Expression of COX2. J Clin Endocrinol Metab. 2015 Sep;100(9):E1197-205. doi: 10.1210/jc.2015-2330. Epub 2015 Jul 20. Bayne RA, Kinnell HL, Coutts SM, He J, Childs AJ, Anderson RA. GDF9 is transiently expressed in oocytes before follicle formation in the human fetal ovary and is regulated by a novel NOBOX transcript. PLoS One. 2015 Mar 19;10(3):e0119819. doi: 10.1371/journal.pone.0119819. eCollection 2015. McLaughlin M, Kinnell HL, Anderson RA, Telfer EE. Inhibition of phosphatase and tensin homologue (PTEN) in human ovary in vitro results in increased activation of primordial follicles but compromises development of growing follicles. Mol Hum Reprod. 2014 Aug;20(8):736-44. doi: 10.1093/molehr/gau037. Epub 2014 May 15. Comish PB, Drumond AL, Kinnell HL, Anderson RA, Matin A, Meistrich ML, Shetty G. Fetal cyclophosphamide exposure induces testicular cancer and reduced spermatogenesis and ovarian follicle numbers in mice. PLoS One. 2014 Apr 1;9(4):e93311. doi: 10.1371/journal.pone.0093311. eCollection 2014. He J, Stewart K, Kinnell HL, Anderson RA, Childs AJ. A developmental stage-specific switch from DAZL to BOLL occurs during fetal oogenesis in humans, but not mice. PLoS One. 2013 Sep 25;8(9):e73996. doi: 10.1371/journal.pone.0073996. eCollection 2013. Childs AJ, Kinnell HL, He J, Anderson RA. LIN28 is selectively expressed by primordial and pre-meiotic germ cells in the human fetal ovary. Stem Cells Dev. 2012 Sep 1;21(13):2343-9. doi: 10.1089/scd.2011.0730. Epub 2012 Mar 6. Childs AJ, Cowan G, Kinnell HL, Anderson RA, Saunders PT. Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. PLoS One. 2011;6(6):e20249. doi:10.1371/journal.pone.0020249. Epub 2011 Jun 3 Fowler PA, Anderson RA, Saunders PT, Kinnell H, Mason JI, Evans DB, Bhattacharya S, Flannigan S, Franks S, Monteiro A, O'Shaughnessy PJ. Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary. J Clin Endocrinol Metab. 2011 Jun;96(6):1754-62. doi: 10.1210/jc.2010-2618. Epub 2011 Mar 23 Childs AJ, Kinnell HL, Collins CS, Hogg K, Bayne RA, Green SJ, McNeilly AS, Anderson RA. BMP signaling in the human fetal ovary is developmentally regulated and promotes primordial germ cell apoptosis. Stem Cells. 2010 Aug;28(8):1368-78. doi: 10.1002/stem.440 Anderson RA, Sciorio R, Kinnell H, Bayne RA, Thong KJ, de Sousa PA, Pickering S. Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy. Reproduction. 2009 Oct;138(4):629-37. doi: 10.1530/REP-09-0144. Epub 2009 Jul 14 Ellen Grünewald, Hazel L Kinnell, David J Porteous, Pippa A Thomson. GPR50 interacts with neuronal NOGO-A and affects neurite outgrowth. Mol Cell Neurosci. 2009 Dec;42(4):363-71. doi: 10.1016/j.mcn.2009.08.007. Epub 2009 Aug 21. This article was published on 2024-07-08
