We talk about macroscopy when describing imaging methods that look at objects visible to the naked eye, i.e. a few cm to mm. This does not mean there will not be any details, just nothing smaller than a single cell. Objects imaged on macroscopes can be a whole organ, a drosophila, zebrafish larvae, eggs, large organoids etc. Expand all Collapse all Optical Projection Tomography (OPT) An OPT images a sample multiple times, rotating the sample by a fraction of a degree between pictures. The resulting file is reconstructed in 3D. Our OPT can image samples in brightfield (greyscale) or fluorescence – GFP/YFP and RFP only – down to a resolution of about 10 micrometres. Your samples must be transparent (cleared) and embedded in agarose before imaging on the OPT to ensure optimal fluorescent imaging. Usage of the OPT is restricted to facility team members. Special applications and features: Greyscale imaging (better for fluorescence) 3D image files The largest object imaged on the OPT is the size of a marble. Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access Nikon AZ100 The Nikon AZ100 is an upright macroscope with three lenses (1x, 2x, 5x) and eight levels of zoom. It is equipped with a 12-bits greyscale camera and can image DAPI, GFP, RFP, and far red. Individual filters in UV, GFP and RFP allow for optimal imaging, but the microscope also has a quadruple filter for automated imaging in all four channels. It has a range of inserts, an automated stage for multiple fields of view and can run z-stacks. This macroscope allows for swan neck and/or bottom illumination in pseudo phase contrast. Special applications and features: Greyscale imaging (better for fluorescence) Automated stage for multi-position, mosaics, and Z stacks Largest field of view on the Nikon AZ100 is 1.3cm wide, but with the 5x lens and 8x zoom we can see single nuclei on organoids. Range of stage inserts, from slides to large petri dish Location: IRR Imaging Hub Contact IRR.Imaging@ed.ac.uk for access Leica M165 FC The Leica M165FC is an upright macroscope with two lenses (1x, 1.6x) and zoom from 0.75x to 12x. It is equipped with a colour camera and can image coloured samples (e.g. X-Gal staining) as well as very bright fluorescent samples in DAPI, GFP, RFP and far red. It can run z-stacks. This macroscope allows for swan neck and/or bottom illumination in pseudo phase contrast. Special applications and features: Colour imaging and Z stacks Largest field of view on the Leica M165FC is 3cm wide, but with the 1.6x lens and 12x zoom, we can see small organoids. Location: First floor of IRR North (CRM Building), opposite the histology lab. Contact IRR.Imaging@ed.ac.uk for access This article was published on 2024-07-08
