Assay core services

Services and equipment for the Assay lab

Institute for Regeneration and Repair (IRR), located within the University of Edinburgh, houses an Assay core service that specialises in optimising and validating immunoassays, running automated analysers for steroid and protein hormones, and biochemistry analytes. Operated by Dr Kirsten Wilson expert advice on sample collection, matrices and volumes required for analysis, quality control and interpretation of results is also available. For costing information see our prices and booking page (EASE login required). 

Equipment

  • Cobas Mira+ (biochemistry)
  • Roche e411 (hormones and proteins)

Sample types

Samples are primarily human or mouse serum or plasma, but analysis of saliva and urine from a range of mammalian species (giant panda, polar bear, elasmobranchs, tiger, koala) and TC media can also be analysed.

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Progesterone and Estrogen metabolites

Hormones

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Molecular diagram of Cortisol and Androgens (Testosterone and DHEA)

Any of the listed steroid hormone reagents that can be purchased for a Roche e411 analyser:

 Visit the Roche website for their assay menu

Additionally, commercially purchased kits can be tested and run by the service at a cost.

Steroid Hormones

  • Progestogens
  • Cortisol
  • Testosterone
  • DHEA/DHEA-S
  • Estrogens (E1G) by Enzyme Immunoassay (EIA).

Protein Hormones

  • LH
  • FSH

Biochemistry Analytes

Liver and kidney function tests are available; albumin, ALP, AST, ALT, bilirubin, urea, creatinine and microalbumin. Other tests include amylase, cholesterol, LDH, triglycerides and glucose, among others. New tests can be added to this repertoire provided reagents and controls are available for automation on a Cobas Mira+.