Some experiments require instruments or techniques that we do not have but are available in other facilities in Edinburgh. This list is not exhaustive. More can be found at the links below. We are happy to direct you to the right imaging system once we know your needs - come and talk to us!Edinburgh Bioimaging website (Facilities and Technologies webpage)College of Medicine and Veterinary Medicine, Shared Research Facilities website (Light Imaging webpage) Superresolution Whilst we have an Elyra, our colleagues of The Edinburgh Super-Resolution Imaging Consortium (ESRIC) are happy to help with other imaging techniques.Superresolution microscope (Elyra), IRR websiteThe Edinburgh Super-Resolution Imaging Consortium (ESRIC) website Light sheet Light sheet for fixed samplesOur colleague at INCR has a series of light sheet microscopes and optimised know-how with sample clearing (link below).Contact cristina.martinez-gonzalez@ed.ac.uk to enquire about sample compatibility and for access.Light-Sheet Microscopy and 3D Analysis Facility (LSM3D) webpage Light sheet for live samplesThe Roslin Institute Imaging facility (link below) offers access to a light sheet that can handle live samples. Contact Anna.Raper@roslin.ed.ac.uk to enquire about sample compatibility and for access.Roslin Bioimaging and Flow Cytometry webpage Electron microscopy 3D electron microscopyA new facility for 3D electron microscopy has been recently set up in the Roslin Institute. Contact jonathan.moss@ed.ac.uk for enquiries and access. Laser tweezers The C-Trap system enables you to trap single molecules of DNA or proteins, manipulate them, and measure changes to their structure with high spatial and temporal resolution. Simultaneously, you can visualize a trapped construct and any interacting proteins in real time. Contact David.Kelly@ed.ac.uk for advice and access Omics-imaging Spatial transcriptomicsWhole transcriptomics on a slide using Visium HD, Xenium, or CosMx SMI. These allow for sequencing-based transcriptomics or measuring targeted RNAs directly on tissue (link below). We have successfully combined Xenium results with multiplex immunostaining from our Phenocycler on the same slide. Single Cell & Spatial Biology IRR webpage Mass spectrometry imagingIdentify molecules on a slide. The MSI (MSI) workflow combines MALDI-based analysis with high-resolution mass spectrometry to map the spatial distribution of metabolites, lipids, and drugs in biological tissues. Contact shazia.khan@ed.ac.uk for enquiries and access. Genomics imagingThe Genetics Core uses an Element AVITI24 platform that combines sequencing chemistry with integrated optical detection to allow spatial detection of RNA, protein and direct in-sample sequencing. For more on what the platform enables, visit the link below.Contact Lee.Murphy@ed.ac.uk for more information.Element Biosciences website This article was published on Monday 8 July 2024
